cloning and expression of functional reteplase in escherichia coli top10

Cloning and Expression of Functional Reteplase in Escherichia coli TOP10

BACKGROUND Production of tissue Plasminogen Activator protein (t-PA) in prokaryotes systems has many problems such as the lack of active protein production, multiple purification steps, and renaturation process which has been shown to be costly and time-consuming. METHODS In this study, reteplase which is the nonglycosylated active domain of t-PA was used to transform TOP10 Escherichia coli (...

expression and activity evaluation of reteplase in escherichia coli top10

reteplase is a part of tissue plasminogen activator (t-pa) used for theremoval of thrombi in blood vessels. in the present study we express the reteplase genein escherichia coli top10 and then its thrombolytic activity was measured. the recombinant plasmid pbadgiiia was transformed into the competent escherichia coli top10 and then transformed bacteria was seeded into bioreactor containing 1.5 ...

expression of reteplase in escherichia coli top10 using arabinose promoter

Optimization of the Expression of Reteplase in Escherichia coli TOP10 Using Arabinose Promoter

BACKGROUND Reteplase is a mutant version of t-PA (tissue plasminogen activator) with prolonged half-life. In the present study, E. coli Top 10 bacteria were utilized in the production of reteplase, which is the nonglycosylated active domain of t-PA. Reteplase gene was ligated into pBAD/gIII plasmid which, allows secretion of this protein in periplasmic space. It would allow the correct formatio...

optimization of the expression of reteplase in escherichia coli top10 using arabinose promoter

conclusions reteplase was expressed in e. coli top 10 after activation of pbad/giiia promoter region by arabinose and optimized. results the obtained recombinant plasmid was sequenced to confirm the presence and correct framing of reteplase gene regarding the expression of reteplase. maximum production of this enzyme was obtained under the following condition: 0.0002% l-arabinose at 37°c for 2 ...

Cloning and Expression of Mannheimia haemolytica PlpE Gene in Escherichia coli and its Immunogenicity Assessment

Mannheimia haemolytica is responsible for considerable economic losses to cattle, sheep, and goat industries in many parts of the world. This bacterium isone of the causative agents of shipping fever in cattle. Current vaccines against M. haemolytica are moderately efficacious since they do not provide complete protection against the disease. Production of a...